Automated determination of amplified PCR products: application to HCV viremia detection and quantification.

Aún no traducido Aún no traducido
Categoría Estudio primario
RevistaCellular and molecular biology (Noisy-le-Grand, France)
Año 1995
A simple and reliable automated method was developed for the detection of amplified products after PCR, which provides an alternative to the time-consuming Southern blotting and hybridization procedure. For the determination and quantification of hepatitis C viremia, the digoxigenin labelling process was applied during the PCR of the amplicons, followed by an inverse hybridization assay performed with biotinylated probes. The detection of the PCR amplified products could be processed as simply as an ELISA, or by means of an automated analyser.
Epistemonikos ID: 56100529a93086b2935fad663757fafbe650f3f6
First added on: Nov 14, 2024