The effect of PKA signaling pathway in processing uii induced myocardial hypertrophy

Objectives: To further explore the role of PKA signaling pathway in UII induced cardiomyocyte hypertrophy. Methods: Isolate and culture the neonatal rat myocardial cells. The primary myocardial cells were randomly divided into 4 groups: (1) the normal control group; (2) the UII 10-8 mol/L group; (3) KT-5720+ UII 10-8 mol/L group; (4) KT-5720 group. Cell planimetric area was viewed using an inverted fluorescent microscope. BCA method was used to detect total intracellular protein in myocar-dial cells and Hoechst 33258 staining to detect total intracellular DNA. Then the protein/DNA ratio was calculated. Fura-3AM fluorescence staining was used to detect myocardial cell cytoplasm Ca2+ concentration. Intracellular P-PKA, PKA, P-PLN, PLN, SERCA2a protein content was measured by Western blot assay. Results: (1) UII induced myocardial hypertrophy in the concentration of 10-8 mol/L. Pretreatment with KT-5720 could inhibit the hypertrophy of large extent. (2) UII could lead to sharp increase in intracellular Ca2+ concentration; K T-5720 pretreatment also attenuated this increase degree. (3) KT-5720 pretreatment significantly increased the expression of P-PKA, P-PLN and SERCA2a protein which were induced decreasing by UII. Conclusion: PKA signaling pathway plays an important role in UII induced hypertrophy of myocardial cells.