THE POTENTIAL OF CURCUMIN AND PENTAGAMAVUNON - 0 AS ANTI VIRAL DENGUE - 2

More than 40% of the world's population who live in tropical and subtropical regions at risk for dengue infection. Specific and effective antiviral therapies to treat dengue infection has not been found yet. Many researches proved that curcumin has preventive activity againts viruses, such as vasicular stomatis (VSV), HSV 1 and 2, parainfluenza -3, reovirus-1, feline corona virus, feline herpes virus. Curcumin also known to perform the inhibition of ubiquitin - proteasome system that decrease the production of Japanese encephalitis in neuroblastoma cells. Pentagamavunon-0 (PGV-0) is expected to have better activity than curcumin. This study aims to determine the cytotoxic effect and the potential of curcumin and PGV-0 as antiviral Dengue-2 on vero cells. Including experimental study. Cytotoxic test performed to obtain a safe concentration of curcumin and PGV-0 on vero cells followed by antiviral test using immunocytochemistry SBPC (Streptavidin Biotin Peroxidase Complex). The results showed that the safe concentrations for curcumin is 6.25 ppm and PGV-0 is 1.5625 ppm based on cytotoxic test to vero cell. The positive rate from Immunocytochemistry test showed that no significant difference between curcumin and PGV-0 treatment. However, when compared with the positive control results are significantly different. We concluded both curcumin and PGV-0 can reduce the positive rate caused Dengue-2 infection at one day incubation.