Clinical evaluation of different detection methods of SARS-CoV-2 IgM/IgG antibody in the COVID-19 diagnosis

Objective To explore the application of different detection methods for IgM and IgG antibodies of new coronavirus (SARS-CoV-2) in 2019 coronavirus disease (COVID-19). Methods Twenty-five patients with COVID-19 were selected, and 20 patients who were excluded from SARS-CoV-2 infection during the same period were used as the control group. Serum SARS-CoV-2 IgM and IgG were detected by magnetic particle chemiluminescence method and colloidal gold method respectively. antibody. Simultaneous detection of serum procalcitonin (PCT), ferritin and C-reactive protein (CRP) in patients with COVID-19. Results The sensitivity of the chemiluminescence method to detect serum SARS-CoV-2 IgM and IgG antibodies was 48% and 56%, and the specificity was 100%. The colloidal gold method was used to detect the sensitivity of serum SARS-CoV-2 IgM and IgG antibodies. They were 88% and 76%, respectively, and the clinical specificity was 100%. The two methods used to detect the serum SARS-CoV-2 IgM and IgG antibodies were 68.9% and 73.3% respectively. Of the 25 COVID-19 patients, 36% had elevated serum PCT, 72% had elevated serum CRP, and 84% had elevated serum ferritin. Conclusion　The detection methods of SARS-CoV-2 IgM and IgG antibodies are quite different, and should be considered comprehensively when used in the clinical diagnosis of COVID-19 patients.