Alb-PRF as an Adjunct to Nonsurgical Periodontal Therapy

This study is designed as a multicenter, randomized, controlled, split‐mouth clinicaltrial with a single‐masked outcome assessment. A total of 24 adults with periodontitisand at least two clinically comparable periodontal sites suitable for a split‐mouthcomparison will be enrolled.Within each participant, eligible periodontal sites will be randomly assigned to acontrol or test condition. Control sites will receive standard nonsurgical periodontaltherapy, including oral hygiene instruction and supra‐ and subgingival mechanicaldebridement. Test sites will receive the same nonsurgical periodontal therapy followed bythe local application of Alb‐PRF into the periodontal pocket. Alb‐PRF will be preparedfrom the participant's own venous blood under sterile conditions during the treatmentvisit.Clinical periodontal assessments will be performed at baseline and at 1, 3, and 6 months.The clinical variables will include periodontal probing depth, clinical attachment level,bleeding on probing, plaque index, and gingival index. The examiner performing theclinical measurements will be masked to the site allocation. The primary outcome measurewill be the change in periodontal probing depth from baseline to 3 months.An integrated microbiological assessment will be conducted in the same participants,without enrolling a separate study population. Subgingival plaque samples will becollected from the randomized control and test sites at baseline and 30 days aftertreatment. The bacterial loads of Porphyromonas gingivalis, Aggregatibacteractinomycetemcomitans, and Prevotella intermedia will be measured using quantitativereal‐time polymerase chain reaction. Changes in bacterial load between baseline and day30 will be evaluated as exploratory outcomes and compared between the control andAlb‐PRF‐treated sites.