Dengue virus-specific antigen lymphoproliferation assay with human T cells

Papers dealing with the study of the immune response to dengue virus infection and with the role it may play in the pathogenesis of dengue hemorrhaghic fever are increasingly important. The memory human T cell response in individuals with history of dengue infection during the Cuban epidemic was studied in this paper for a further evaluation of the antigenicity of viral proteins. To this end, mononuclear cells of peripheral blood from individuals immune to dengue and from a group of control subjects with viral antigens were incubated. It was obtained a significant proliferative response of lymphocytes from individuals with history of infection against dengue virus type 2 compared with control subjects. It was proved this way the dengue virus-specific memory T CD4+ cell response in the individuals under study.