A highly selective and sensitive Tb[3+]-acetylacetone photo probe for the assessment of acetazolamide in pharmaceutical and serum samples.

A novel, simple, sensitive and selective spectrofluorimetric method was developed for the determination of Acetazolamide in pharmaceutical tablets and serum samples using photo probe Tb[3+]-ACAC. The Acetazolamide can remarkably quench the luminescence intensity of Tb[3+]-ACAC complex in DMSO at pH 6.8 and λex = 350 nm. The quenching of luminescence intensity of Tb[3+]-ACAC complex especially the electrical band at λem = 545 nm is used for the assessment of Acetazolamide in the pharmaceutical tablet and serum samples. The dynamic range found for the determination of Acetazolamide concentration is 4.49 × 10[-9]-1.28 × 10[-7] mol L[-1], and the limit of detection (LOD) and limit of quantification (LOQ) are (4.0 × 10[-9] and 1.21 × 10[-8]) mol L[-1], respectively.