Studies on Yeast Vector System for Eukaryotic Gene Cloning -New Cloning Vector Containing Autonomous Replicating Segment from Candida tropicalis-

Category Primary study
Year 1987
Autonomous replicating segment (ARS) derived from Candida tropicalis IFO 0518 has been newly cloned in Saccharomyces cerevisiae YNN27 with YIp5. A new plasmid which named as pIKS was isolated from S. cerevisiae YNN27 transforment. The cloned gene contained a 8kb-Sal I fragment of C. tropicalis DNA (C. Sars). We have compared the stability of pIKS with that of YRp7 in S. cerevisiae YNN 27 and established a restriction cleavage sites with various endonucleases.
Epistemonikos ID: bdecf4f15f5a231130ce44e4e15b1c9a810e358c
First added on: Feb 03, 2021