Evaluating the Benefits of Spring season Cleansing Therapy through emesis (vasantik vaman) on Metabolism, Inflammation, and Gut Health

INTERVENTION: CONDITION: PRIMARY OUTCOME: Changes in the Gut and oral microbiota composition assessed through qPCR and 16S rRNA amplicon sequencing and metabolite profiling by liquid chromatography‐mass spectrometryTimepoint: Day 0, Days 3, Day 6/8/10, Day 8/10/12, Day 11/15/19, Day 30, and Day 180 SECONDARY OUTCOME: Change in Complete Hemogram, Lipid profile, LFT, RFT, Bicarbonate, Fasting InsulinTimepoint: Day 0, after Snehpana, after Vamana, Day 180) Change in fasting adiponectin and leptinTimepoint: Days 0, 30, 60 and 180 Change in Fasting GlucoseTimepoint: Day 0, After Vaman, Day 180 Change in HbA1C, homeostatic model assessment to quantify insulin resistance (HOMA‐IR), Vit B12, Vit DTimepoint: Day 0, Day 180 Change in Quality of Life assessed through SF‐36Timepoint: Baseline, Days 30, 60, 180 Change in urine F2‐isoprostanes as a biomarker for oxidative stress by specific ELISA testTimepoint: Day 0, Days 3, Day 6/8/10, Day 8/10/12, Day 11/15/19, Day 30, and Day 180 Change in urine metabolome from baselineTimepoint: Day 0, after Snehana, after vamana, Day 30 Reported AE/ADRTimepoint: Participant reported AE/ADR recorded in structured formats INCLUSION CRITERIA: Healthy male and female volunteers in the age group 18‐45 years. In case of female, 48 hours after menses stop BMI‐ 18.5‐ 24.9 kg/m2 Participants devoid of any acute or chronic diseases as determined by medical history, physical examination and laboratory tests. BMI In the range of 18.5 to 24.9 kg/m2 Participants deemed fit for Vamana Karma, as per Ayurveda classical texts. Willing to participate for a period of 06 months and adhere to the study interventions and advocacies throughout the intervention period