estradiol administration for patients with a history of poor IVFresponse

INTERVENTION: Intervention 1: Women in interventional group (n=55) receive 2 mg estradiol twice a day is started on luteal day 21 and Subsequently stimulation is initiated with gonadotropin in cycle day 2 and 0.25 mg cetrorelix is administrated daily when at least one follicle reach 13‐14 mm and continue until the day of hCG injection. 10000 IU hCG is administered IM when at least two follicles reach >17 mm, followed 36 hours later by oocyte retrieval. Embryos are transferred on the third day after retrieval. Intervention 2: women In control group (n=55) is received Buserelin 50 µg twice/day in cycle day 2 and after two days gonadotropin stimulation is started. Buserelin continue until the day of hCG injection. 10000 IU hCG is administered IM when at least two follicles reach >17 mm, followed 36 hours later by oocyte retrieval. Embryos are transferred on the third day after retrieval. Treatment ‐ Drugs women In control group (n=55) is received Buserelin 50 µg twice/day in cycle day 2 and after two days gonadotropin stimulation is started. Buserelin continue until the day of hCG injection. 10000 IU hCG is administered IM when at least two follicles reach >17 mm, followed 36 hours later by oocyte retrieval. Embryos are transferred on the third day after retrieval. Women in interventional group (n=55) receive 2 mg estradiol twice a day is started on luteal day 21 and Subsequently stimulation is initiated with gonadotropin in cycle day 2 and 0.25 mg cetrorelix is administrated daily when at least one follicle reach 13‐14 mm and continue until the day of hCG injection. 10000 IU hCG is administered IM when at least two follicles reach >17 mm, followed 36 hours later by oocyte retrieval. Embryos are transferred on the third day after retrieval. CONDITION: female infertility female infertility. ; female infertility PRIMARY OUTCOME: The number of oocyte retrival. Timepoint: the day of puncture. Method of measurement: counting by microscope. SECONDARY OUTCOME: Chemical pregnancy. Timepoint: 14 days after embryo transfer. Method of measurement: serum BHCG. INCLUSION CRITERIA: INCLUSION CRITERIA: history of poor response to stimulation (<3 dominant follicles or estradiol <500pg/ml), basal FSH level>10 mIU/ml,antral follicle count <5. Exclusion criteria: endometriosis,only one ovary, endocrine or metabolic disorder.