Evaluation of the effectiveness of NSF treatment of incipient dental caries

INTERVENTION: Intervention1: Nano Silver Fluoride: Silver Nitrate (for Preparation of Silver Nano Particle, Having Anti‐ microbial Efficacy) ‐ Sodium Borohydride (Reducing agent in Formation of Silver Nano Particle) ‐ Sodium Fluoride (Enhancement of Mineralization, Inhibition of Bacterial Activity in Plaque) ‐ Chitosan (Synergism action of Chitosan, Silver Nanoparticles and Fluoride as Antimicrobial) For experimental group Nano Silver Fluoride(NSF), tooth will isolate with cotton roll. Selected tooth will dried with cotton pellet. NSF (33,989.8 m/ml) solution will apply on the tooth surface for 2 min. Each tooth received two drops of NSF with a micro brush, equivalent to a dose of 10 mg of solution. For the control group selected tooth will isolate with cotton roll. One drop of tap water with fluoride concentration in 1 ppm will apply on the tooth surface (another active carious lesion of same patient) for 2 min. Follow up will be done by same examiner at 7 days, 5 and 12 month interval. Control Intervention1: WATER: AS TAP WATER WHICH STUDENTS USE TO DRINK COINTAIN 1 PPM OF FLUORIDE SO SELECT AS A CONTROL GROUP CONDITION: PRIMARY OUTCOME: ; The anticaries effect of Nano Silver Fluoride will be evalluate on inciepnt caries lesion. ; The sample size was calculated by using confidence ninety five percent and power eighty percent the sample size was sixty one. ; The sample size was increased to make up possible losses and to improve accuracy. ; Based on the test material via split mouth design the 176 teeth were divided into two groups. ; Group I Nano Silver Fluoride 88 teeth.Group II Water 88 teeth.Timepoint: evalluation of caries lesion will be done after application after 7 days, 5month and 12 months SECONDARY OUTCOME: NILTimepoint: NIL INCLUSION CRITERIA: 6‐7 YR GIRLS AND BOYS MEDICALLY HEALTHY CHILDREN DECIDUOUS TEETH WITH TWO CARIES LESION AT ENAMEL LEVEL