Molecular beacon for detection of MERS-CoV and uses thereof

Authors
Category Primary study
Year 2015
The present invention relates to a molecular beacon for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV). More specifically, the present invention relates to a molecular beacon for detecting the nsp3 region of the MERS-CoV and the M base sequence region which is a structural protein region, a kit for the diagnosis of a MERS-CoV infection using the same, and a method for detecting MERS-CoV. The molecular beacon of the present invention has a target affinity higher than the affinity of an antibody and is much smaller in size than an antibody and an aptamer so that the beacon can react with a target molecule with a high binding force. In addition, the molecular beacon is not sensitive to temperature change, has no denaturation, and has the advantage of enabling detection with only a small amount of target nucleic acid molecules within a short time. Furthermore, since detection is possible by a simple method using fluorescence or UV spectrum, a kit for detecting MERS-CoV using the molecular beacon of the present invention can be widely used for the diagnosis of a MERS-CoV infection.
Epistemonikos ID: 94189fa07b7ad841aae5604cde9ad0bcdbd3cd5e
First added on: Aug 13, 2020