Effect of Plant Hormones on Micro-propagation of Tashnedari (Scrophularia striata) Medicinal plant

Tashnedari (Scrophularia striata) is native to Iran and its main habitat is Illam province, that indiscriminate harvesting is placed it on the list of endangered medicinal plants. In order to micro-propagation and resolve its endangered experiment was done in biotechnology laboratory of sari agricultural and natural resources university in September 2012. Effect of BAP, 2, 4-D and NAA hormones in MS medium was evaluated on callusing and micro propagation of this plant. Explants were prepared from stem. Factorial experiment were done as completely randomized design with three replications. Results showed that the combination of (BAP 1.5 mg/l and 2, 4-D 1.5 mg/l) was the best treatment for callusing ( = 52.22) and the combination of (BAP 0.5 mg/l and NAA 0.05 mg/l) was the best treatment for regeneration ( = 43.33). Callus fresh weight was measured for shoot explant. And hormonal combination of BAP (3.5mg/l) and 2, 4-D (1.5 mg/l) had the highest increase in fresh weight of callus (1.09 g). Key word: Tshnedari, Callus induction, Explant, Regeneration