Demonstration of the nutritional benefits of following an organic vs. conventional diets using “omics technologies”

INTERVENTION: Participants are randomised to one of two groups who consume each of the two study diets in a random order. There is a 1.5 month wash‐out period between the two dietary interventions during which participants can consume their usual diets. Intervention A: Participants consume a healthy and organic diet for 28 days Intervention B: Participants consume a healthy and conventional diet for 28 days Both interventions follow a very similar dietetic pattern, a healthy diet (typical Mediterranean diet) rich in vegetables foods. Biological samples (blood, urine, stool and saliva) will be taken at the beginning and end of each intervention (baseline and 28 days). The urine recollection will be for 24 hours before of the test. The blood will be centrifuged and all the samples will be stored at ‐80ºC until analysis. CONDITION: Diet ; Nutritional, Metabolic, Endocrine PRIMARY OUTCOME: 1. Carotenoids in plasma and saliva is measured using HPLC‐DAD at the start and end of each of the 28 day intervention periods; 2. Bioavailability, identification and quantification polyphenols in stools, plasma, urine and saliva are assessed using LTQ‐Orbitrap Mass Spectrometry and HPLC‐MS/MS at the start and end of each of the 28 day intervention periods; 3. Pesticides in plasma are measured using GC‐MS at the start and end of each of the 28 day intervention periods SECONDARY OUTCOME: 1. Adherence to the Mediterranean diet is measured using a questionnaire designed for the purpose of this study at the start and end of each of the 28 day intervention periods; 2. Food intake is measured using food frequency questionnaires designed for the purpose of this study at the start and end of each of the 28 day intervention periods; 3. Physical activity is evaluated with the Minnesota Leisure Time Physical Activity questionnaire at the start and end of each of the 28 day intervention periods; 4. Urine metabolites (metabolomics) are assessed using LTQ‐Orbitrap Mass Spectrometry at the start and end of each of the 28 day intervention periods; 5. Markers of inflammation are measured by inmunnoassay on plasma samples at the start and end of each of the 28 day intervention periods; 6. Constituency of gut microbiota is measured using LTQ‐Orbitrap Mass Spectrometry on stool samples at the start and end of each of the 28 day intervention periods INCLUSION CRITERIA: 1. Healthy volunteers 2. Males and females 3. Age: 18‐40