Reagent for detecting COVID-19 through chemiluminescence immunoassay and detection method thereof

The invention provides a reagent for detecting COVID-19 through chemiluminescence immunoassay and a detection method thereof. An indirect process is used, a COVID-19 antigen is coated on a magnetic microsphere, the anti-human IgG/IgM is labeled with acridinium ester, and the acridinium ester is excited by the excitation liquid to emit light so as to detect the COVID-19 antibody, and the detectionmethod combining the acridinium ester or acridinium sulfonamide compound labeling technology and the superparamagnetic nano-microsphere labeling technology is adopted; the superparamagnetic nano-microsphere labeling technology is a technology of coating nano-microspheres with antigens or antibodies, and the superparamagnetic nano-microsphere labeling technology can enlarge the reaction surface area by 10 to 50 times and accelerate the reaction efficiency; the reaction process is suspended in a reaction solution and belongs to homogeneous reaction, so that the reaction speed can be increased; connection belongs to chemical connection of bioactive substances, so that the coating efficiency is higher, and the coating is more stable in a liquid environment. The detection reagent disclosed by the invention is high in specificity, high in sensitivity, simple and convenient to separate, rapid, non-toxic, safe and stable, short in detection time by adopting the reagent, high in efficiency andgood in stability.