Regenerative Medicine Advancements: A Systematic Review on the Combinatory Effect of Platelet-Rich Plasma/Fibrin and Collagen

BACKGROUND: The regeneration of injured tissues remains a major clinical challenge. Among emerging biomaterials, collagen with platelet-rich plasma (PRP) or platelet-rich fibrin (PRF) showed promising outcomes, individually and in combination. OBJECTIVE: To systematically review clinical evidence on the efficacy, applications, and safety of PRP/PRF and collagen for regenerative medicine applications. METHODS: A systematic literature search was conducted in PubMed, Wiley Online Library, Google Scholar, and ClinicalTrials.gov (search date: September, 2025). Inclusion criteria: clinical studies evaluating PRP/PRF and collagen formulations. Exclusion: preclinical only or nonoriginal research. Data were synthesized narratively. RESULTS: Twenty-six clinical studies were included. Applications included gingival recession, periodontitis, tendon injuries, bone regeneration, peripheral nerve repair, and chronic ulcers. Most studies reported positive outcomes, though many lacked control groups or had small sample sizes. No serious adverse events were reported. CONCLUSION: PRP/PRF and collagen show potential for various clinical applications in regenerative medicine. However, randomized clinical studies are necessary to demonstrate their superiority to standard treatment and to standardize protocols.