Effect of garlic allicin and vitamin C on muscle soreness exercise-induced

INTERVENTION: garlic allicin intake 70 mg/pday for 16 days Intervention 1: vitamin C intake 1000 mg/pday for 16 days. Intervention 2: garlic allicin intake 70 mg/pday for 16 days. Intervention 3: placebo intake for 16 days. Intervention 4: run on downhill treadmill with the gradient of ‐%5 at %75 of heart rate reserve (HRR) for 45 min. Placebo placebo intake for 16 days Prevention run on downhill treadmill with the gradient of ‐%5 at %75 of heart rate reserve (HRR) for 45 min vitamin C intake 1000 mg/pday for 16 days CONDITION: Delayed onset muscle soreness. ; Soft tissue disorder, unspecified Soft tissue disorder, unspecified PRIMARY OUTCOME: Serum creatine kinase enzyme. Timepoint: before intervention, on 14 days post intervention and before exercise, 1, 24, 48 hours after 14 days intervention and exercise. Method of measurement: blood sample. Serum lactate dehydrogenase enzyme. Timepoint: before intervention, on 14 days post intervention and before exercise, 1, 24, 48 hours after 14 days intervention and exercise. Method of measurement: blood sample. SECONDARY OUTCOME: Rate of perceived exertion (evaluate muscle soreness). Timepoint: before intervention, on 14 days post intervention and before exercise, 1, 24, 48 hours after 14 days intervention and exercise. Method of measurement: score of borg’s scale. INCLUSION CRITERIA: INCLUSION CRITERIA: physical health. Exclusion criteria: family history of cardiovascular defects, family history of pulmonary and renal defects, antioxidant supplement consumption including vitamin C, E,.., anti‐inflammation drugs consumption.