Effects of Kangliyin on the levels of serum antibody in the chick model of respiratory coronavirus infection

AIM:To study the effects of Kangliyin compound (KLY,the compound formulation of a Chinese herbal medicine has 15 kinds of ingredients,including artemisia apiacea,baical skullcap root,tangerine peel,pinellia tuber,frutus aurantii immaturus,caulis bambusae in taeniis,tuckahoe,the larva of silkworm with botrytis,turmeric,agastache,rhubarb,cicada ecdysis,and so on.) treatment on the infectious bronchitis virus (IBV) infection models and to evaluate its effects on the levels of IBV antibody in the serum. METHODS:Seven-day-old chicks were inoculated with the respiration type of IBV M41 strain. All the chicks were bred in a low temperature and highly moisturized controlled environment. The model group and normal control group were fed with sterile normal saline,while the three treatment groups were administrated with KLY compound. Positive control group was irrigated with ganciclovir. These feeds were lasted for 5 d. The clinical symptoms and the pathological changes of all groups' bronchus and lung tissues were examined. The levels of IBV antibody in the serum in each group were also checked by using ELISA method at the end of the study. RESULTS:Compared with the model group,KLY compound significantly increased the serum titer of IBV antibody in the three treatment groups (P