First report on in vivo fertility trial of frozen thawed boar semen in India

The present study was conducted to evaluate the in vivo fertility of frozen thawed boar semen. Twenty ejaculates collected from six mature boars were frozen in a programmable freezer. After freezing the semen was evaluated for different sperm quality parameters. Twenty five sows were inseminated artificially utilizing frozen thawed semen. The percentage of sperm motility, live sperm, live intact acrosome, plasma membrane intact sperm, HOST-reacted sperm, live sperm with high mitochondrial membrane potential, lipid peroxidised sperm and DNA-damaged sperm of frozen semen utilised for AI were 56.25 +/- 0.96, 63.75 +/- 1.47, 59.88 +/- 1.09, 41.08 +/- 1.01, 40.31 +/- 1.02, 86.23 +/- 1.29, 9.28 +/- 0.83 and 4.20 +/- 0.29 respectively. The farrowing rate was 44.00 per cent and the mean litter size at birth was 5.91 +/- 0.69. It could be concluded that the freezing and insemination protocol of boar semen used in the present study resulted in moderate fertility of frozen boar semen and it would help in further improvement and utilization of frozen boar semen for AI in India.