The prospective exploratory study of safety and efficacy on the patients post-penetrating keratoplasty of topical tacrolimus 0.1%

INTERVENTION: Target drug: tacrolimus 0.1% ophthalmic susupension Control: artificial tear (My tear eye drop) CONDITION: Penetrating keratoplasty (PKP) PRIMARY OUTCOME: Rejection rates of the graft on week 52 post‐operatively. SECONDARY OUTCOME: 1) Survival rates of the graft on week 52 post‐operatively; "2) Survival rates of the graft on week 26, 52 and last observation post‐operatively ; 3) Adverse drug reactions on week 26, 52 and last observation post‐operatively; 4) Best corrected visual acuity; 5) Corneal endthelial cell density; 6) Influenced factors to survival rate of the graft on week 52 post‐operatively; 7) Survival rates of the graft when adjustment of influence factors; 8) Recruitment of dendritic cell and reconstitution of trigerminal" INCLUSION CRITERIA: "Patients with PKP who are relevant the following conditions 1) Patients with the history of PKP 2) Patients with the history of rejection 3) Patients with vascuralization in corneal stroma (more than half) 4) Atopic patients with karatoconus "