The effect of dietary rapeseed (canola) versus olive oil supplementation on serum lipids, liver enzymes and postprandial inflammatory responses in adipose tissue in obese men.

INTERVENTION: Eligible individuals were randomized into two treatments to receive a four week daily supplementation with either 50 g of rapeseed oil or 50 g of olive oil. At the end of the supplementation individuals underwent a biopsy of subcutaneous adipose tissue at fasted conditions at 08:30 am, followed by a test meal of 835 kcal and an additional biopsy of subcutaneous adipose tissue four hours after the test meal. CONDITION: Obesity ; Nutritional, Metabolic, Endocrine ; Obesity PRIMARY OUTCOME: To assess the influence of a daily nutritional supplementation with either 50 g of rapeseed oil or 50 g of olive oil to an isocaloric diet on body composition, serum lipids, serum liver enzymes and inflammatory gene expression in sub cutaneous adipose tissue. Body composition will be determined using an air‐displacement plethysmography system and serum lipids and liver enzymes will be measured using standard techniques in a certified laboratory at the German Institute of Human Nutrition before and four weeks after dietary intervention. Inflammatory gene expression in sub cutaneous adipose tissue will be measured four weeks after dietary intervention using quantitative real time‐PCR. SECONDARY OUTCOME: To assess the influence of a daily nutritional supplementation with either 50 g of rapeseed oil or 50 g of olive oil to an isocaloric diet on plasma n‐6 and n‐3 PUFA content and plasma n‐6/n‐3 ratio before and four weeks after dietary intervention. n‐6 and n‐3 PUFA content will be determined after lipid extraction from plasma using thin layer chromatography and gas chromatography. The plasma n‐6/n‐3 ratio will be calculated. INCLUSION CRITERIA: 1. Males, age between 18 and 65 years 2. Obesity, BMI between 27‐35 kg/m²