The effects of acute power and aerobic exercise on bone metabolism in healthy young individuals

INTERVENTION: We will examine the effects of a single 60 minute exercise session (aerobic or power exercises) on plasma uncarboxylated osteocalcin (unc‐OC), adionectine (AN). Blood samples will be taken before exercise, immediately after and at 0.5, 1, 2, 24 and 48 hours post exercise. CONDITION: bone metabolism PRIMARY OUTCOME: Uncarboxylated osteocalcin (unc‐OC) will be analysed based on a sandwich type electro‐chemiluminescence immunoassay (ECLIA) utilizing anti‐OCG3 mouse monoclonal antibody labelled with Ruthenium (Ru) which luminates with electric stimuli. Magnetic beads bound to anti GluOC 4‐5 mouse monoclonal antibody is used as a solid phase. After binding the unc‐OC in the sample with the GluOC4‐5 bound beads and Ru labelled OCG3, an electric impulse is applied via an electrode. The level of luminescence for the Ru complexes bound to solid phase antibody reflects the amount of unc‐OC in the sample. The concentration of the unc‐OC in the sample is measured by comparing the sample's luminescence to that of a calibrated solution of known unc‐OC standards SECONDARY OUTCOME: Adiponectin will be measured by ALPCO assay (ALPCO Diagnostics, Salem, NH). INCLUSION CRITERIA: non‐active, healthy young volunteers