Viral Titer Quantification of West Nile Virus by Immunostaining Plaque Assay.

Authors
Category Primary study
JournalMethods in molecular biology (Clifton, N.J.)
Year 2023
Immunostained plaque assay based on the specific antibody binding to viral antigen enables the detection and titration of virus infectivity, especially for viruses that could not form plaques using the classical crystal violet or neutral red staining methods. Here we describe the application of this method to quantify viral titers of wild-type West Nile virus (WNV-WT) and replication-defective WNV-ΔNS1 virus.
Epistemonikos ID: 38ea7691b1f08b5145bf73497512be15083b57c8
First added on: Nov 05, 2022