Combined Effect of Prolotherapy and Personalized Physical Activity on Knee Osteoarthritis Progression

The aim of this clinical trial is to assess whether prolotherapy combined with personal physical activity is effective in managing pain (measured using the VAS and WOMAC scales) and improving the quality of life in participants with Grade 2 Knee Osteoarthritis (OA). This trial will also measure the levels of IL-1β and MMP-3 in the participants. The primary research questions are: 1. Does prolotherapy combined with personal physical activity reduce pain levels and improve clinical outcomes in participants with knee OA compared to those receiving prolotherapy ? 2. How does this intervention affect the levels of IL-1β and MMP-3 in participants? Researchers will compare prolotherapy combined with personal physical activity to prolotherapy alone to determine its impact on clinical outcomes and changes in biomarkers in patients with knee OA.