The Effects of Tyrosol and Creatine on Exercise Performance and Cognition in Healthy Adults

On the day of testing, the investigators will instruct subjects to avoid high‐intensity,muscle‐damaging exercise for a minimum of 72‐hours prior to the visit, and avoid caffeineand alcohol 12 hours prior to each study visit, and during the testing visit itself.After a minimum 8‐hour overnight fast, subjects will proceed with a series of tests inthe following successive order: (1) Vital Signs (heart rate, blood pressure), followed bythe POMS and Subjective Vitality Scale (SVS). (2) DXA for body composition, followed byconsumption of a standardized food bar snack, and at least a 15‐minute break after thesnack. After the 15‐minute break, subjects will proceed with the following tests: (3)Digit Span Test (DST) and Stroop Test (in that successive order), which have previouslydemonstrated to be sensitive to creatine supplementation; (4) full‐body strengthmeasurement using a computerized mid‐thigh pull; (5) Bench press, starting withdetermination of the subject's 5RM (repetition maximum) bench press, which will be usedto calculate their 1RM. After five‐minutes of rest, subjects will then perform 4‐sets ofbench to failure at 50 % of 1RM with a 1‐minute rest between all sets. The investigatorswill calculate a fatigue curve from the successive 4‐sets. Importantly, the weight willbe kept identical at baseline and 8‐weeks to help control for training intensity, andsubjects will repeat the identical warm up procedure at 8‐weeks as done at baseline tohelp control for load. To capture shifts in total repetitions as a primary outcome,participants will be directed to reach failure on the final set of the 5RM at 8 weeks aswell as all sets performed at 50% of 1RM during both baseline and 8‐week assessments; (6)after an 10‐min break, subjects will then complete a time‐to‐exhaustion endurancetesting protocol (graded cycle test). Rate of Perceived Exertion (RPE) will also becollected during the endurance protocol, using the Borg RPE scale at each stage. (7)Finally, after a 5‐min break, subjects will repeat the DST and Stroop Tests at the endof the session, followed by the SVS Scale.After the 8 week supplementation period, all baseline measures will be repeatedfollowing baseline criteria (in the same successive order; Visit 2). Additionally, likertscales for soreness will be taken prior to DXA. Additionally, following the DXA, butprior to cognitive testing on post‐testing onsite days (Visit 2), a full dosage of thestudy treatment will be taken with water, as well as a standardized snack which will beadministered on‐site by staff. The subjects will wait for at least 15 minutes after thetreatment to begin the remaining tests. The likert scale for soreness will again be takenon its own at Visit 3 and 4 (Approximately 24 and 48 hours later). Additionally, subjectswill be instructed to take one final serving of their treatment at a normal time of dayon Visit 3.