An electrochemical nano-genosensor for SARS-CoV-2 detection utilizing Ce-metal organic framework, dendritic palladium nano-structure, and sulfur-doped graphene oxide.

The emergence of COVID-19 has underscored an urgent demand to develop an innovative, rapid, and reliable diagnostic tool for early detection of the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). Biosensors present a viable alternative, offering reliability, precision, and cost efficiency that address the limitations of current molecular and serological detection methods, thus facilitating timely identification of COVID-19. In this study, a novel nano-genosenor platform fabricated using advanced nanomaterials based on Ce-metal organic framework (Ce-MOF), dendritic palladium nano-structure (Den-PdNS), and sulfur-doped reduced graphene oxide (S-rGO) for detection of RNA-dependent RNA polymerase (RdRp) SARS-CoV-2 gene targets. The fabricated nano-genosensor represents a remarkable limit of detection (LOD) of 0.2 fM, a proper sensitivity of 10.067 μA cm-2, and a wide linear range from 10 fM to 10 μM. The nano-genosensor's performance was assessed in real saliva samples, demonstrating robust recovery and accuracy, even in complex biological environments, underscoring its potential for medical applications. The fabricated nano-genosensor exhibited proper selectivity, repeatability, and reproducibility in detection of SARS-CoV-2.