Combined of 8-Week Bifidobacterium Supplementation With Training Ameliorate the Anaerobic Exercise Capacity of Cross-Country Skiers

Objectives: Use the targeted metabolomic analyses of plasma to explore the effect of 8‐week different dose of Bifidobacterium supplementation combined with training on the anaerobic exercise capacity of cross‐country skiers. Methods: Sixteen male cross‐country skiers who volunteered to participate in the experiment were recruited and randomly divided into low dose group (L, count 1×108CFU) and high dose group (H, count 1×109CFU). Supplementary scheme is 4 times/day (with the three meals and at 21:00 in the evening), 6 days/week, for eight weeks. The experiment was conducted in a double‐blind mode. The dietary and energy consumption of the subjects were recorded every week. Blood and feces were collected before and after the experiment. Body composition, anaerobic capacity, blood routine and feces targeted metabolomics were measured. Results: (1) After 8 weeks, there are no significant difference of the dietary intake, energy consumption and body composition. (2) Mean corpuscular hemoglobin concentration of both L (331.6±5.2 g/L vs 341.5±5.0 g/L, P<0.01) and H (329.6±4.6 g/L vs 340.3±6.4 g/L, P<0.01) group were significantly increased. (3) During the Wingate Test, blood lactic acid on timepoints of 15 s (10.3±3.3 mmol/L vs 7.4± 1.1 mmol/L, P<0.05) and 30 s (13.0±1.9 mmol/L vs 11.0± 1.7 mmol/L, P<0.05) of L group were significantly decreased. (4) Correlation analysis find mean corpuscular hemoglobin concentration is positively correlated with L‐2‐aminobutyric acid, citraconic acid, glyceric acid and N���Methyl‐L‐glutamic acid in blood. Conclusions: 8‐week Bifidobacterium supplementation may help reduce the production of blood lactic acid during anaerobic exercise and increase the average hemoglobin concentration of cross‐country skiers. Funding Sources: The National Key R&D Program of China.