Comparison Efficacy of Intraarticular Knee Injection in Knee Osteoarthritis With Leukocyte-rich and Leukocyte-poor Platelet-rich Plasma

INTERVENTION: Experimental Drug,Experimental Drug,Active Comparator Drug Leukocyte‐rich platelet‐rich plasma,Leukocyte‐poor platelet‐rich plasma,Corticosteroid Peripheral blood centrifuge at 300 G for 5 min, separate the plasma and buffy coat layers, and then centrifuge at 700 G for 17 min with calcium chloride at the ratio of 1:5 to PRP by volume. ,Centrifuge at 123 G for 15 min, separate the plasma and buffy coat layers, and then centrifuge at 448 G for 10 min. Calcium chloride was added 1:5 to PRP by volume.,1 ml of triamcinolone acetonide (40mg/ ml) mixed with 0.9% NaCl to a total volume of 4 ml CONDITION: Osteo Arthritis Knee Osteoarthritis of the knee ; Osteo Arthritis Knee PRIMARY OUTCOME: WOMAC 6 months The Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) questionare SECONDARY OUTCOME: VAS pain 6 months Visual analogue scale for pain,the time up and go test 6 months stands up upon therapist command walks 3 meters, turns around, walks back to the chair and sits down,30‐second chair stand test unit second 6 months rise to a full standing position, then sit back down again. repeat for 30 seconds INCLUSION CRITERIA: Age greater than or equal to 50 years old, Plain radiographs knee AP shows Kellgren‐Lawrence classification 1‐4, Refuse the use of NSAIDs for 2 weeks.