The effect of Astaxanthin on the expression of genes involved in WNT/ß-catenin pathway in granulosa cells and inflammatory factors in the serum of PCOs patients candidate for ART

Authors
Category Primary study
Registry of TrialsIranian Registry of Clinical Trials
Year 2023
INTERVENTION: Intervention 1: Astaxanthin group: In this group, patients will receive a 6 mg astaxanthin tablet daily for two months after eating. Intervention 2: Control group: In this group, patients will receive a 6 mg placebo tablet daily for two months after eating. CONDITION: E28.2 Poly cystic ovarian syndrome. ; Polycystic ovarian syndrome PRIMARY OUTCOME: Measuring the expression level of Wnt/ß‐catenin signaling pathway genes in patients' granulosa cells. Timepoint: Two months after the start of astaxanthin. Method of measurement: Real time PCR test. Measuring the level of expression of the phosphorylated and dephosphorylated form of beta‐catenin protein. Timepoint: Two months after the start of astaxanthin. Method of measurement: Western blot test. Measurment of serum cytokine levels in patients with polycystic ovary syndrome. Timepoint: At the beginning of the study before the start of the intervention and two months after the start of astaxanthin. Method of measurement: ELIZA. SECONDARY OUTCOME: Checking the quality of oocytes. Timepoint: Two months after taking astaxanthin. Method of measurement: Observation with inverted microscope. Checking the rate of fertilization fertilization. Timepoint: Two months after taking astaxanthin. Method of measurement: Observation with microscope. INCLUSION CRITERIA: Diagnosis of Poly cystic ovary syndrome based on Rotterdam criteria People are in the age range of 18 to 40 years
Epistemonikos ID: 26a1dc7525c7ee8908f830ae10b770486e24889f
First added on: Feb 20, 2024