'Monty's Surprise' apple Bioavailability study

INTERVENTION: The study will be conducted in Te Ohu Health unit at Massey University, Palmerston North. This study will be performed as a single‐blind, randomised cross over study. One group (Group A) of the participants (6 participants) will receive placebo meal first and second group (Group B) (6 participants) will receive study meal first. Each study visit will last 8 hours.There will be one week wash out period between two visits. All the study meals will be prepared in the product development lab, Massey University, Palmerston North. The study meal is MS apple puree (500g). The apple puree will be provided as a breakfast. Macronutrient matched placebo will be prepared from water and sugars presented in apple puree. The trial will consist of a screening visit and 2 study visits (visit 1 and visit 2). The night before the study visits, participants will be asked to fast from 10 pm (overnight) till the start of the study (8 am). At the study visit a cannula will be inserted into their forearm through which the blood samples will be collected by by trained and approved individual. CONDITION: Cancer ‐ Any cancer Cancer; ; Cancer Diet and Nutrition ‐ Other diet and nutrition disorders PRIMARY OUTCOME: Antioxidant activity of phytochemicals presented in plasma after apple puree consumption.[0,1,2,4,6,8 hours post study meal consumption] Plasma metabolites[0,1,2,4,6,8 post study meal consumption] SECONDARY OUTCOME: Glucose levels evaluated from plasma at 0, 15, 30 minutes and 1,2 hours after apple puree consumption.[0, 15 and 30 minutes, 1, 2 hours post study meal consumption] INCLUSION CRITERIA: • non‐smokers • age 18‐45 • healthy with normal body mass inde X(BMI from 18 kg/m2 to 25 kg/m2) • no more than 10 hours of vigorous exercise per week • able to consume apple • for women before menopause • able to not drink coffee/tea for 24 hours