Comparison of sequence analysis and a novel discriminatory real-time PCR assay for detection and quantification of Lamivudine-resistant hepatitis B virus strains.

Category Primary study
JournalJournal of clinical microbiology
Year 2004
We report a rapid and accurate real-time PCR-based method to quantify wild-type and lamivudine-resistant hepatitis B virus by using a common forward primer paired with different reverse primers. Excellent concordance was demonstrated between sequencing and the discriminatory real-time assay; however, a mixture of quasispecies was more frequently detected by discriminatory real-time PCR.
Epistemonikos ID: 1c80eebf61eede988a5248ff05c70a6dcf9a2083
First added on: Sep 18, 2024