The acute effect of red wine and on Cytochrome p450 metabolites of arachidonic acid

INTERVENTION: Men who drink>30g alcohol/day and <110g alcohol/day will participate in a randomised 3 period crossover design. The study will compare drinking 375ml of red wine, or the same volume of de‐alcoholised red wine or water over a 30 min period with food. The 3 beverages will be administered in random order in a crossover design on 3 separate days. The 3 treatment days will be 2 weeks apart. During the 2 week intervals between treatment days volunteers will maintain their normal dietary and alcohol intake. Prior to each treatment day volunteers will abstain from drinking alcohol for 48 hrs, and will fast for 12hrs to before being studied. On each of the 3 treatment days, volunteers will have blood pressure (BP) monitored for 24hr. On each treatment day, cytochrome P450 metabolites of arachidonic acid will be measured in urine and in plasma and cell membranes before and after 2hrs, 4hr and 24hr of drinking the beverage . CONDITION: cardiovascular disease PRIMARY OUTCOME: Changes in cytochrome P450 metabolites of arachidonic acid in urine, plasma cell membranes, between the treatments SECONDARY OUTCOME: Changes in ambulatory Blood pressure between the treatmants INCLUSION CRITERIA: alcohol consumption >30g/day <110gm/day, non‐smokers, not taking medication