The effect of probiotics on the absorption of essential nutrients from plant protein

INTERVENTION: Co‐administration of 20 g of protein (vegetable protein isolated from yellow pea (Pisum Sativum), NUTRALYS® S85F, Roquette Freres S.A., France) with either 10 billion CFU of a multi‐strain probiotic (5 billion CFU L. paracasei LP‐DG® (CNCM I‐1572) and 5 billion CFU L. paracasei LPC‐S01 (DSM 26760), AminoAltaâ„¢, SOFAR S.p.A., Italy) or a placebo (maltodextrin, Glucidex® 12, Roquette Freres S.A., France). The study consisted of a two week supplementation period, followed by a four week washout, followed by a crossover of groups and another two weeks of supplementation. As for the randomization process, we used Random.org to randomize everyone as they came into the lab. We assigned the A treatment as 1 and the B treatment as 2 and the website gave us a number for that person. CONDITION: Healthy individuals ; Not Applicable PRIMARY OUTCOME: Plasma amino acid concentrations measured using tandem‐mass spectrometry (LC/MS/MS) at baseline, 30, 60, 120, and 180 minutes post‐ingestion SECONDARY OUTCOME: Adverse events throughout the study measured using case report forms INCLUSION CRITERIA: 1. Healthy men between the ages of 18–35 years 2. Normal body weight (body mass index (BMI) of 19 – 24.99 kg/m²) 3. Recreationally active (according to American College of Sports Medicine Guidelines) 4. Subjects were not allowed to consume any nutritional or ergogenic supplement known to affect measures of the current study for the prior six weeks, including probiotics, prebiotics and digestive enzymes