Effects of acute supplementation with Whey Protein and Collagen on the appetite in humans

INTERVENTION: Dietary supplement Dietary Supplements The research was a clinical trial, randomized, crossover, double‐blind and controlled placebo, with the participation of 10 healthy female volunteers, aged between 18 and 35 years old. The research evaluated the degree of hunger, satiety, appetite, nausea and hormonal markers regulating appetite in the blood before and after eating whey protein or hydrolyzed collagen. The volunteers participated in two groups, with a week between tests: ‐ Whey Protein Group (GW): n = 10 participants who received a sachet containing 40g of concentrated whey protein (containing 160kcal, 25g of protein, 10g of carbohydrates and 2.3g of lipids, diluted in 200ml of filtered water with 50ml of juice passion fruit concentrate ‐ Collagen Group (GC): n = 10 participants who received 1 sachet of hydrolyzed collagen totaling 40.3g (26g of protein, 10g of natural flavored maltodextrin, 2.3g of refined oil, 2g of vanilla flavoring and 0.001g of sucralose , diluted in 200 ml of filtered water with 50 ml of concentrated passion fruit juice. CONDITION: Diabetes Mellitus Obesity Obesity; Diabetes Mellitus ; Obesity ; Diabetes Mellitus PRIMARY OUTCOME: Evaluate whether supplementation with whey protein will increase satiety in women SECONDARY OUTCOME: Assess whether supplementation with whey protein compared to hydrolyzed collagen can: ; ‐Increase serum insulin secretion ; ; 408/5000 ; Approximately 10 mL of blood were collected and separated into two tubes, containing EDTA. The blood was rotated in a refrigerated centrifuge (Hitachi CF16RN, Hitachinaka, Ibaraki, Japan) at 4000 rpm, for 10 minutes at 4 ºC. The plasma was stored in plastic eppendorf tubes and stored in a freezer at –80 ºC (Panasonic MDF‐ U56VC‐PA, Mexico) ; The colorimetric enzymatic method was used for analysis Assess whether supplementation with whey protein compared to hydrolyzed collagen can: ; ‐Reduce appetite; ; Assess whether supplementation with whey protein compared to hydrolyzed collagen can: ; ‐Reduce plasma fasting blood glucose concentrations; ; ; 408/5000 ; Approximately 10 mL of blood were collected and separated into two tubes, containing EDTA. The blood was rotated in a refrigerated centrifuge (Hitachi CF16RN, Hitachinaka, Ibaraki, Japan) at 4000 rpm, for 10 minutes at 4 ºC. The plasma was stored in plastic eppendorf tubes and stored in a freezer at –80 ºC (Panasonic MDF‐ U56VC‐PA, Mexico) ; The colorimetric enzymatic method was used for analysis INCLUSION CRITERIA: The study population consisted of women with a Body Mass Index (BMI) above 18.5 kg / m2 and less than 25.5 kg / m2 and aged between 18 and 35 years.