Prenylflavanoids as a biomarker of beer consumption

INTERVENTION: Male volunteers: Intervention 1: Administration of 330 mL beer (14.5 g ethanol) Intervention 2: Administration of 660 mL beer (29 g ethanol) Intervention 3: Administration of 990 mL beer (43.5 g ethanol) Female volunteers: Intervention 1: Administration of 330 mL beer (14.5 g ethanol) Intervention 2: Administration of 495 mL beer (21.7 g ethanol) Intervention 3: Administration of 660 mL beer (29 g ethanol) CONDITION: Beer consumption ; Nutritional, Metabolic, Endocrine PRIMARY OUTCOME: Concentrations of urinary prenylflavanoids will be determined by liquid chromatography coupled to tandem mass spectrometry (LC?MS/MS). These determinations will be carried out in first morning urine samples collected the day before the first intervention and in the subsequent mornings following each intervention. Creatinine adjustment will be used to normalize analyte concentrations in these urine samples. SECONDARY OUTCOME: No secondary outcome measures INCLUSION CRITERIA: Young healthy volunteers of age 28±3 years, body mass index 22.69±2.69 kg/m2