Formation of bilirubin photoisomers in optimalized phototherapy of neonatal jaundice.

INTERVENTION: Intensified phototherapy with single fluorescent unit (one Bilicompact light) vs Double fluorescent unit (double bilicompact. light) vs phototherapy with Photodiodes (Neoblue). The neoBLUE unit emits blue LED light in the 450‐470 nm with peak at 468 nm spectrum. This range corresponds to the bilirubin peak absorption wavelength of 458 nm. BiliCompact contains ten 9W,12.7‐cm length fluorescent bulbs (BAM/PL9/52, Ralutec 9W/71 G 23). These bulbs have broad Gaussian emission spectra roughly from 400 ‐525 nm with a peak at 450 nm and intense mercury emission lines at 405, 436, and 546 nm Arm 1: Single phototherapy provided by one unit neoBLUE `Trademark` Arm 2: Single phototherapy with single unit BiliCompact.`Trademark`. Arm 3: Double phototherapy was administered by two units BiliCompact`Trademark`. We will take blood sample to analyse bilirubin photoisomer right befor starting phototherapy, after 30 min.60 min, 120 min Duration of phototherapy depends on the patient's response to phototherapy and how quickly falls serum total bilirbin. For this study, all children receive phototherapy at least 2‐4 hours, because we are interested to see how quickly formed fotoisomerer after 15 min, 30min, 60 min, 120 min and 240 min.and 240 min. during phototherapy. CONDITION: Neonatal jaundice PRIMARY OUTCOME: Formation of bilirubin isomers. ; Stereoisomers of bilirubin formed during phototherapy. ; We will measure these photoisomers by HPLC method. HPLC ( High‐performance Liquid Chromaatography) for analyse of photoisomer.W will measure photoisomers who formed at 0 min, 15 min, 30 min., 60 min, 120 min & 240 min. ; HPLC method: Bilirubin isomer were analyzed on a Dionex ultimate 3000 system , including a multiple wavelength detector ( Dionex, Sunnyvale, CA). The collumn used for HPLC was Kinetex core‐shell 2,6 um C‐18 ( 15 x0,46 cm). SECONDARY OUTCOME: Duration of phototherapy. ; Measuring the time of phototherapy. INCLUSION CRITERIA: Neonates with jaundice. ( Only neonate with jaundiice with BW > 1000 g)