Effect of vitamin C on the expression of collagen IV alpha 1 mRNA in rats with diabetic nephropathy

Aim: To observe the Effect of vitamin C on the expression of Collagen IV α1 mRNA and renal function in rats with diabetic nephropathy. Methods: The experiment was carried out in the animal section of Nanhua University from January to April 2004. The rats were fasted for 12 hours after 1-week pre-feeding, and then they were treated with intraperitoneal injection of 60 mg/kg streptozotocin-citromalic acid buffer and citromalic acid buffer of the same volume (control group) respectively to establish models of type 1 diabetes mellitus according to the standards that uric glucose after 72 hours was +++ to ++++ and the venous blood glucose was ≥16.7 mmol/L. The SD rats were randomly divided into normal control group, diabetic group and vitamin C group, and the fluctuation of blood glucose was kept around 25 mmol/L for 16 weeks. During and after the treatment, the general state, blood glucose levels, blood urea nitrogen, serum creatinine, clearance rate of creatinine, and 24-hour urinary albumin excretion rate were observed, and the type the gene expression of collage IV α1 gene in the kidney tissue was determined by in situ hybridization analysis. Results: All t he 30 SD rats were involved in the analysis of results without death. 1 As compared with the normal control group, the body mass was obviously decreased, uric amount was increased, blood glucose was elevated, and there were significant differences (t=4.945, 3.779, P < 0.05). After treatment, the body mass and renal mass/body mass in the vitamin C group were significantly increased as compared with those in the diabetic group (t=2.927, 30.32, P < 0.05). 2 As compared with the normal control group, the serum urinary albumin excretion rate, serum creatinine and blood urea nitrogen were obviously increased, and clearance rate of creatinine was significantly decreased (t=4.113,3.125 1,2.798,P < 0.05). Compared the diabetic group with the vitamin C group, the serum urinary albumin excretion rate, serum creatinine and blood urea nitrogen were obviously increased, and clearance rate of creatinine was decreased (t=2.875, 2.994,3.102, P < 0.05). 3 As compared with the normal control group, the expression of renal glomerular extracellular matrix collage IV α1 gene was significantly up-regulated, and the determination of gray value showed significant differences between the two groups (t=4.766, P < 0.05); After treatment, the pigmenting of renal tissue was light and sparse, and the relative expression of collage IV α1 gene was obviously down-regulated as compared with that in the diabetic group (t=2.879, P < 0.05). Conclusion: Vitamin C has no effect in decreasing the level of blood glucose, but plays a certain role in protecting kidney.