Photoluminescence and Surface Plasmon Resonance to Study Bacteria Biofilm Supported on Gold Surface for Anti-E.Coli Antibodies Detection

In the present paper, we reported a new biosensing approach using photoluminescence technique for antibodies detection using bacteria biofilm supported on gold surface as bioreceptor. The proposed approach offer both qualitative and quantitative power photoluminescence signal. The photoluminescence spectra shows closed correlation with the antibodies injected concentrations, while the wavelength shift is proved to be dependent of the chemical charge nature of the gold/electrolyte interface. Furthermore, the antibody detection was tested with surface plasmon resonance set-up for sensitivity comparison. These two techniques show higher sensitivity for real time detection with an obtained detection limit of 10-6µg.mL-1 and 10-7µg.mL-1 for photoluminescence and surface plasmon resonance set-up respectively.