Multiplex PCR-RFLP ensayo para la detección del factor V Leiden y la protrombina G20210A.

Autores
Categoría Estudio primario
RevistaGenetic testing
Año 2004
Factor V Leiden and prothrombin G20210A are clinically relevant genetic risk factors for venous thrombosis. Molecular diagnostic testing for factor V Leiden and prothrombin G20210A is widespread, and laboratories use a variety of technical approaches. Here we introduce a multiplex polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) based on single (Mn/l) restriction endonuclease digestion. The assay was shown to simultaneously and accurately detect factor V Leiden and prothrombin G20210A mutations.
Epistemonikos ID: 58681cc66df0c5371506541612d64e7282f6f1f6
First added on: Jun 08, 2011